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NON-FatPowderedMilk ，≥99%
畅销产品

D6340G
RT
4年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Guangzhou Shenyang Quantity Cart
D6340G 100g $13.76
D6340G 500g $49.54
Product Name NON-FatPowderedMilk
Alias DifcoTM Skim Milk
Appearance (character) 类白色或黄色
Purity： ≥99%
Introduction： 以下仅供参考具体请根据实验要求调整:
试用说明介绍
使用建议（以Western Blot为例）
配方与步骤 :
封闭液配方：5% 脱脂奶粉+ TBST（Tris缓冲盐溶液 + 0.1% Tween-20）。
例如：5 g奶粉 + 100 mL TBST，充分溶解后过滤（避免颗粒残留）。
封闭条件：
室温1小时或4℃过夜（时间过长可能增加背景）。
关键优化点浓度测试：尝试3%~5%奶粉，避免过度封闭导致信号减弱。
抗体兼容性：
若背景高，换用BSA封闭液（尤其磷酸化抗体）。
某些抗体（如抗生物素抗体）可能与奶粉成分交叉反应。
洗涤加强：封闭后用 TBST洗3次×5分钟，减少残留。

These protocols are for reference only. Biotopped does not independently validate these methods.

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