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Tris BHF DNA提取酚试剂(tris平衡酚)PH＞7.8,非无菌
畅销产品

TH0025G
4℃避光
1年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TH0025G 250ml $24.77
Product Name
Appearance (character) 淡黄色液体
Introduction：

开封后保存: 4℃，避光保存，请尽可能的在3个月内用完。
产品说明:
本产品仅用于科研，不可用于其他。
本试剂是用Tris-HCl（pH8.0）饱和过的DNA提取酚试剂，为浅黄色透明液体，有刺激性气味，上层为Tris盐酸缓冲液，pH>7.8，下层内加有抗氧化的8-羟基喹啉。本品适用于分离DNA。
使用方法:
组织裂解液，确保pH为8.0左右(可加入0.1体积的1M Tris-HCl，pH8.0，将其调为8.0左右)，再加入0.5体积的DNA提取酚试剂（使用时静置，取下层使用。），0.5体积核酸提取液（24：1），振荡10秒钟后冰浴15分钟。离心后取水相（上层）加等体积异丙醇沉淀DNA，75%乙醇漂洗1次，微干燥后溶于适量TE中备用。
注意事项:
(1) 应尽量在通风橱内使用，避免接触或吸入。
(2) 如发现变为红色或棕色，表明已发生氧化，不能继续使用。
(3) 为了您的安全和健康，请穿实验服并戴一次性手套操作。

These protocols are for reference only. Biotopped does not independently validate these methods.

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