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10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free)
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Catalog No.： Top0033S

Alias： Show

Storage Condition： RT

Shelf Life： 1年

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
Top0033S	500ml	$12.38

Product Name	10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free)
Introduction：	产品简介：平衡盐溶液(Balanced Salt Solution，BSS)与细胞生长状态下的 pH 值、渗透压等环境状态一致，具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用，可满足体外实验中细胞生存并维持一定的代谢的基本需要。 BBS 配方常有改动，如 Hank's BBS 由不含钙镁或酚红的，也有含钙镁含酚红的等等, Dulbecco's PBS 有不含钙镁或含钙镁的等。HBSS、EBSS、PBS 等都是与较弱的磷酸盐缓冲液相关的盐溶液。常见的平衡盐溶液有 Eargle's 液、Hank's 液、磷酸盐缓冲溶液(PBS)等。磷酸缓冲盐溶液(10×PBS,无钙镁)即 Phosphate-Buffered Saline，是最常用的磷酸盐缓冲溶液之一，又称 Dulbecco's 磷酸缓冲盐溶液、Dulbecco's PBS、D-PBSA、CMF-DPBS或 D-PBS、DPBS 溶液, 主要由氯化钠、氯化钾、磷酸氢二钠、磷酸二氢钾组成。操作步骤 (仅供参考) ： 1、进行细胞培养过程中细胞的洗涤时，按 10×PBS:无菌水=1:9 进行稀释，即取 10×PBS 1份、无菌水 9 份，充分混匀，即获得 PBS 工作液。 2、进行常规实验（不要求无菌）时，按 10×PBS:双蒸水或去离子水=1:9 进行稀释，即取10×PBS 1 份、双蒸水或去离子水 9 份，充分混匀，即获得 PBS 工作液。注意事项： 1、在进行细胞培养过程中细胞的洗涤时，应注意无菌操作，避免被微生物污染。 2、本溶液其盐离子浓度很高，易产生少许沉淀，出现该种情况后，一般置于温浴至沉淀溶解即可，如果产生沉淀较多应弃用。 3、该试剂经高压灭菌处理。

Product Name

10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free)

Introduction：

产品简介：
平衡盐溶液(Balanced Salt Solution，BSS)与细胞生长状态下的 pH 值、渗透压等环境状态一致，具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用，可满足体外实验中细胞生存并维持一定的代谢的基本需要。 BBS 配方常有改动，如 Hank's BBS 由不含钙镁或酚红的，也有含钙镁含酚红的等等, Dulbecco's PBS 有不含钙镁或含钙镁的等。HBSS、EBSS、PBS 等都是与较弱的磷酸盐缓冲液相关的盐溶液。常见的
平衡盐溶液有 Eargle's 液、Hank's 液、磷酸盐缓冲溶液(PBS)等。
磷酸缓冲盐溶液(10×PBS,无钙镁)即 Phosphate-Buffered Saline，是最常用的磷酸盐缓冲溶液之一，又称 Dulbecco's 磷酸缓冲盐溶液、Dulbecco's PBS、D-PBSA、CMF-DPBS或 D-PBS、DPBS 溶液, 主要由氯化钠、氯化钾、磷酸氢二钠、磷酸二氢钾组成。

操作步骤 (仅供参考) ：
1、进行细胞培养过程中细胞的洗涤时，按 10×PBS:无菌水=1:9 进行稀释，即取 10×PBS 1份、无菌水 9 份，充分混匀，即获得 PBS 工作液。
2、进行常规实验（不要求无菌）时，按 10×PBS:双蒸水或去离子水=1:9 进行稀释，即取10×PBS 1 份、双蒸水或去离子水 9 份，充分混匀，即获得 PBS 工作液。

注意事项：
1、在进行细胞培养过程中细胞的洗涤时，应注意无菌操作，避免被微生物污染。
2、本溶液其盐离子浓度很高，易产生少许沉淀，出现该种情况后，一般置于温浴至沉淀溶解即可，如果产生沉淀较多应弃用。
3、该试剂经高压灭菌处理。

These protocols are for reference only. Biotopped does not independently validate these methods.

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10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free)
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10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free) 畅销产品

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HeadquartersBeijing

Online Service

Customer service Tel:

Technical service Tel：

10X PBS Buffer (0.1 M, pH 7.2-7.4, Calcium and Magnesium Free)
畅销产品