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CTAB抽提液(2*CTAB提取缓冲液) 非无菌
畅销产品

Catalog No.： Top0789

Storage Condition： RT避光

Shelf Life： 2年

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Guangzhou	Shenyang	Quantity	Cart
Top0789	500ml	$33.02

Product Name

Introduction：

产品组成：

名称	规格	保存
2×CTAB提取缓冲液	500 ml	RT
还原剂	1 ml	RT

产品说明：
CTAB (hexadecyltrimethylammonium bromide，十六烷基三甲基溴化铵)，是一种阳离子去污剂，具有从低离子强度溶液中沉淀核酸与酸性多聚糖的特性。在高离子强度的溶液中(>0.7mol/L NaCl) CTAB与蛋白质和多聚糖形成复合物而不沉淀核酸。通过有机溶剂抽提，去除蛋白、多糖、酚类等杂质后加入乙醇沉淀即可使核酸分离出来。
2×CTAB提取缓冲液成分：2%(w/v)CTAB，100mM Tris (pH 8.0)，20mM EDTA，1.4M NaCl，0.2%(v/v) 还原剂(随用随加) 。
操作步骤（仅供参考）：

2×即用型CTAB提取液配制：
按照终浓度0.2% （v/v）加入还原剂，如100ml 2×CTAB提取缓冲液中加入0.2 ml 还原剂。加入还原剂后的2×即用型CTAB提取液4℃贮存。
取0.2-0.5克新鲜植物材料，于液氮中研磨成粉末。
将粉末转入预冷的1.5ml离心管中，立即加入0.7 ml 2×即用型CTAB提取缓冲液，65℃水浴30分钟，间歇混匀。
加入等体积的酚：氯仿：异戊醇(25:24:1)，轻缓颠倒离心管混匀8-10次，常温12000 rpm 离心10分钟。
将上清液转入另一1.5ml离心管中，加入等体积酚：氯仿：异戊醇(25:24:1)，颠倒离心管混匀8-10次，常温12000 rpm离心10分钟。
将上层水相转入新的1.5 ml离心管中，加入0.6倍体积的冰冷异丙醇，轻柔混匀，冰浴30分钟。
12000 rpm4℃ 离心10分钟。
去上清液，加入1ml 70%乙醇漂洗沉淀，4℃12000 rpm 3分钟，吸弃乙醇。
4℃ 12000 rpm 1分钟，用移液器吸尽残余乙醇，超净台吹干沉淀。

注：1.沉淀不能彻底干燥，否则不好溶解。
2.加入30-50μl的超纯水或TE缓冲液溶解DNA，-20℃保存备用。

These protocols are for reference only. Biotopped does not independently validate these methods.

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CTAB抽提液(2*CTAB提取缓冲液) 非无菌
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CTAB抽提液(2*CTAB提取缓冲液) 非无菌 畅销产品

Related Products

HeadquartersBeijing

Online Service

Customer service Tel:

Technical service Tel：

CTAB抽提液(2*CTAB提取缓冲液) 非无菌
畅销产品