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Calcein AM /PI细胞双染试剂盒

Catalog No.： Top9268T

Storage Condition： -20℃避光

Shelf Life： 18个月

Bulk Inquiry? Manual Download Collection Product

Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
Top9268T	100T	$165.12

Product Name
Introduction：	钙黄绿素-AM (Calcein-AM) 和碘化丙啶 (PI) 溶液，分别对活细胞和死细胞染色，可用于同时对活细胞和死细胞进行荧光染色。Calcein-AM的乙酸甲基酯亲脂性很高，使其可透过细胞膜。尽管Calcein-AM本身并不是荧光分子，但通过活细胞内的酯酶作用，Calcein-AM能脱去AM基，产生的Calcein能发出强绿色荧光 (激发: 490 nm，发射: 515 nm)。因此Calcein-AM仅对活细胞染色。另一方面，作为核染色染料的PI不能穿过活细胞的细胞膜。它穿过死细胞膜的无序区域而到达细胞核，并嵌入细胞的DNA双螺旋从而产生红色荧光 (激发: 535 nm，发射: 617 nm)。由于Calcein和PI-DNA都可被490 nm激发，因此可用荧光显微镜同时观察活细胞和死细胞。用545 nm激发，仅可观察到死细胞。由于不同细胞系的最佳染色条件不同，我们建议个别确定Calcein-AM和PI的合适浓度。

Product Name

Introduction：

钙黄绿素-AM (Calcein-AM) 和碘化丙啶 (PI) 溶液，分别对活细胞和死细胞染色，可用于同时对活细胞和死细胞进行荧光染色。Calcein-AM的乙酸甲基酯亲脂性很高，使其可透过细胞膜。尽管Calcein-AM本身并不是荧光分子，但通过活细胞内的酯酶作用，Calcein-AM能脱去AM基，产生的Calcein能发出强绿色荧光 (激发: 490 nm，发射: 515 nm)。因此Calcein-AM仅对活细胞染色。另一方面，作为核染色染料的PI不能穿过活细胞的细胞膜。它穿过死细胞膜的无序区域而到达细胞核，并嵌入细胞的DNA双螺旋从而产生红色荧光 (激发: 535 nm，发射: 617 nm)。由于Calcein和PI-DNA都可被490 nm激发，因此可用荧光显微镜同时观察活细胞和死细胞。用545 nm激发，仅可观察到死细胞。由于不同细胞系的最佳染色条件不同，我们建议个别确定Calcein-AM和PI的合适浓度。

These protocols are for reference only. Biotopped does not independently validate these methods.

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Calcein AM /PI细胞双染试剂盒

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