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DO Supplement –Trp–Ura (DO Dropout Supplement without Tryptophan and Uracil)​

DO2-07
4℃
3年
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
DO2-07 20g $68.52
Product Name DO Supplement –Trp–Ura (DO Dropout Supplement without Tryptophan and Uracil)​
Introduction:

DO(缺陷氨基酸)类培养基的配制 

1. 在 1L 去离子水中加入相应量的 DO 培养基,然后再加入 26.7g Minimal SD Base,搅拌溶解; 

2. 调节 pH 至 5.8,121℃高压灭菌 15min; 

3. 4℃冰箱避光储存已经灭菌的液体培养基。 或:1. 在 900ml 去离子水中加入相应量的 DO 培养基,然后再加入 6.7g YNB,搅拌溶解; 

2. 调节 pH 至 5.8,121℃高压灭菌 15min; 

3. 4℃冰箱避光储存已经灭菌的液体培养基; 

4. 使用时再加入 100ml 已灭菌的 20%葡萄糖。 

These protocols are for reference only. Biotopped does not independently validate these methods.

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