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5-Carboxy-tetramethylrhodamine N-succinimidyl ester ，≥95%

R00014R
5-Carboxy-tetramethylrhodamine N-succinimidyl ester; 4-Carboxytetramethylrhodamine N-succinimidyl ester,5-TAMRA; 5-TAMRA,SE; 5-Carboxytetramethylrhodamine succinimidyl ester; 4-Carbox 5-Carboxy-tetramethylrhodamine N-succinimidyl ester; 4-Carboxytetramethylrhodamine N-succinimidyl ester,5-TAMRA; 5-TAMRA,SE; 5-Carboxytetramethylrhodamine succinimidyl ester; 4-Carbox Show
150810-68-7
-20℃充氮
3年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
R00014R 5mg $79.81
Product Name 5-Carboxy-tetramethylrhodamine N-succinimidyl ester
Alias 5-Carboxy-tetramethylrhodamine N-succinimidyl ester; 4-Carboxytetramethylrhodamine N-succinimidyl ester,5-TAMRA; 5-TAMRA,SE; 5-Carboxytetramethylrhodamine succinimidyl ester; 4-Carbox
CAS No. 150810-68-7
Molecular Formula C29H25N3O7
Molecular Weight 527.52
Purity： ≥95%
Introduction： 溶解建议和操作注意事项指南(以下仅供参考具体请根据实验要求调整)：
溶解方法与建议浓度
溶解性：
1.常用溶剂
有机溶剂：可溶于 DMSO（二甲基亚砜）、DMF（N,N-二甲基甲酰胺）、乙腈等极性有机溶剂。
水溶性：虽然部分资料提到可溶于水，但因该试剂为疏水性荧光染料，建议优先使用有机溶剂溶解后，再以缓冲液（如PBS或HEPES）稀释至工作浓度，以避免析出。
2.溶解建议
配制母液时，推荐使用 DMSO 或 DMF 溶解至 1-10 mM 的浓度（例如：1 mg 粉末溶于约 190 μL DMSO）。
溶解后需分装避光保存于 -20°C，避免反复冻融。

配比方法：
1.标记反应条件
标记对象：适用于含伯胺基团（如赖氨酸侧链）的生物分子（蛋白质、多肽、寡核苷酸等）。
反应摩尔比：推荐 染料:生物分子 = 5-10:1（根据分子量调整），过量染料需通过透析或脱盐柱去除。

反应条件：
pH 8.0-9.0 的缓冲体系（如碳酸氢钠缓冲液或硼酸盐缓冲液）。
室温避光反应 1-2小时 或 4°C过夜（低温可减少副反应）。
2.操作注意事项
避光操作：全程需在暗室或使用铝箔包裹容器，防止荧光淬灭。
分装保存：溶解后的母液建议分装成小份，避免多次冻融导致酯基水解失效。
湿度控制：取用固体时需在干燥环境中操作，防止吸潮。

These protocols are for reference only. Biotopped does not independently validate these methods.

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