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α-淀粉酶(α-AL)检测试剂盒 (微量法)

Catalog No.： TOPEK190792

Storage Condition： RT/4℃

Shelf Life： 6个月

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
TOPEK190792	100T/48S	$74.30

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Introduction：	正式测定前务必取2-3个预期差异较大的样本做预测定测定意义：淀粉酶主要负责催化淀粉水解，包含α-淀粉酶与β-淀粉酶两类。其中α-淀粉酶（EC3.2.1.1）可随机作用于淀粉分子中的α-1,4-糖苷键，水解生成葡萄糖、麦芽糖、麦芽三糖及糊精等还原糖，同时降低淀粉体系粘度，因此也被称作液化酶。测定原理：本试剂盒利用β-淀粉酶的热不稳定性，将粗酶液置于70℃条件下钝化15min，可特异性去除β-淀粉酶活性，使体系中仅保留耐高温α-淀粉酶的催化功能；α-淀粉酶催化淀粉水解生成的还原糖，可与3,5-二硝基水杨酸发生还原反应，生成具有特征吸收的棕红色产物，该产物在540nm波长下有明显吸收峰。通过测定540nm处吸光度的变化，即可计算出α-淀粉酶的活性。

Product Name

Introduction：

正式测定前务必取2-3个预期差异较大的样本做预测定

测定意义：

淀粉酶主要负责催化淀粉水解，包含α-淀粉酶与β-淀粉酶两类。其中α-淀粉酶（EC3.2.1.1）可随机作用于淀粉分子中的α-1,4-糖苷键，水解生成葡萄糖、麦芽糖、麦芽三糖及糊精等还原糖，同时降低淀粉体系粘度，因此也被称作液化酶。

测定原理：

本试剂盒利用β-淀粉酶的热不稳定性，将粗酶液置于70℃条件下钝化15min，可特异性去除β-淀粉酶活性，使体系中仅保留耐高温α-淀粉酶的催化功能；α-淀粉酶催化淀粉水解生成的还原糖，可与3,5-二硝基水杨酸发生还原反应，生成具有特征吸收的棕红色产物，该产物在540nm波长下有明显吸收峰。通过测定540nm处吸光度的变化，即可计算出α-淀粉酶的活性。

These protocols are for reference only. Biotopped does not independently validate these methods.

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α-淀粉酶(α-AL)检测试剂盒 (微量法)

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