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HiPer“死活敏”SYTO9/PI 双染试剂盒(快速区分死活细胞细菌,流式检测）

Catalog No.： TOPEK191223

Storage Condition： -20℃避光

Shelf Life： 1年

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
TOPEK191223	40T	$770.28

Product Name
Introduction：	产品简介：活细菌/死细菌双染试剂盒（SYTO 9/PI Live/Dead Bacterial Double Stain Kit）是⼀款⽅便且操作简单的试剂盒，利⽤SYTO 9绿⾊核酸染料和碘化丙啶（PI）红⾊荧光核酸染料来进⾏细菌活⼒的检测，适⽤于⼤量的细菌种属，包括蜡样芽孢杆菌、枯草芽孢杆菌、产⽓荚膜杆菌、⼤肠杆菌、肺炎克雷伯⽒菌、草分枝杆菌、绿脓杆菌、⾦⿈葡萄球菌、奥拉尼堡沙⻔⽒菌、宋内志贺⽒菌和化脓性链球菌。本试剂盒的⼯作原理在于：SYTO 9和PI的光谱特征以及穿透健康细菌细胞的能⼒不同。单独使⽤时，SYTO 9能对群体内的所有细菌进⾏标记—具有完整膜和受损膜的细菌；相反，PI只能渗透进⼊受损的膜，PI的插⼊会引起SYTO 9染⾊荧光的降低，当体系内加⼊两种染料时。因此，通过适量⽐例的SYTO 9和PI的混合染⾊，具有完整膜结构的细菌呈绿⾊荧光，⽽具受损膜结构的细菌呈红⾊荧光。两者染料的最⼤激发和发射波⻓分别是480/500nm（SYTO 9）和490/635nm（PI）。背景基本⽆荧光。本试剂盒兼容于荧光显微镜，荧光光度计、荧光酶标仪、流式细胞仪或其它荧光检测仪器。

Product Name

Introduction：

产品简介：

活细菌/死细菌双染试剂盒（SYTO 9/PI Live/Dead Bacterial Double Stain Kit）是⼀款⽅便且操作简单的试剂盒，利⽤SYTO 9绿⾊核酸染料和碘化丙啶（PI）红⾊荧光核酸染料来进⾏细菌活⼒的检测，适⽤于⼤量的细菌种属，包括蜡样芽孢杆菌、枯草芽孢杆菌、产⽓荚膜杆菌、⼤肠杆菌、肺炎克雷伯⽒菌、草分枝杆菌、绿脓杆菌、⾦⿈葡萄球菌、奥拉尼堡沙⻔⽒菌、宋内志贺⽒菌和化脓性链球菌。

本试剂盒的⼯作原理在于：SYTO 9和PI的光谱特征以及穿透健康细菌细胞的能⼒不同。单独使⽤时，SYTO 9能对群体内的所有细菌进⾏标记—具有完整膜和受损膜的细菌；相反，PI只能渗透进⼊受损的膜，PI的插⼊会引起SYTO 9染⾊荧光的降低，当体系内加⼊两种染料时。因此，通过适量⽐例的SYTO 9和PI的混合染⾊，具有完整膜结构的细菌呈绿⾊荧光，⽽具受损膜结构的细菌呈红⾊荧光。两者染料的最⼤激发和发射波⻓分别是480/500nm（SYTO 9）和490/635nm（PI）。背景基本⽆荧光。本试剂盒兼容于荧光显微镜，荧光光度计、荧光酶标仪、流式细胞仪或其它荧光检测仪器。

These protocols are for reference only. Biotopped does not independently validate these methods.

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HiPer“死活敏”SYTO9/PI 双染试剂盒(快速区分死活细胞细菌,流式检测）

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