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乙酰辅酶A(Acetyl-CoA)含量测定试剂盒(微板法)

TOPEK191169
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TOPEK191169 96样 $1098.05
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Introduction： 乙酰辅酶A是能源物质代谢的重要中间代谢产物，在体内能源物质代谢中是一个枢纽性的物质。糖、脂肪、蛋白质三大营养物质通过乙酰辅酶A汇聚成一条共同的代谢通路--三羧酸循环和氧化磷酸化，经过这条通路彻底氧化生成二氧化碳和水，释放能量用以ATP的合成。它也是合成脂肪酸、酮体、胆固醇及其衍生物等生理活性物质的前体物质。 苹果酸脱氢酶可催化苹果酸和NAD+生成草酰乙酸和NADH。柠檬酸合酶可催化乙酰辅酶A和草酰乙酸生成柠檬酸和辅酶A。利用苹果酸脱氢酶和柠檬酸合酶的偶联反应，乙酰辅酶A含量和NADH的生成量成正比，340nm下吸光值的上升量反应了乙酰辅酶A含量的高低。

These protocols are for reference only. Biotopped does not independently validate these methods.

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