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植物OsSnRK1A蛋白(OsSnRK1A-Thr177)ELISA Kit

TOPEL03971
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2-8℃
6个月
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEL03971 96T $261.44
TOPEL03971 48T $231.17
Product Name
Introduction：

检测范围：                                                          

1ng/L -50ng/L

使用目的：

本试剂盒用于测定植物组织，细胞上清及相关液体样本中OsSnRK1A蛋白（OsSnRK1A-Thr177）的含量。

实验原理:

本试剂盒应用双抗体夹心法测定标本中植物OsSnRK1A蛋白（OsSnRK1A-Thr177）水平。用纯化的植物OsSnRK1A蛋白（OsSnRK1A-Thr177）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入OsSnRK1A蛋白（OsSnRK1A-Thr177），再与HRP标记的OsSnRK1A蛋白（OsSnRK1A-Thr177）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的OsSnRK1A蛋白（OsSnRK1A-Thr177）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中植物OsSnRK1A蛋白（OsSnRK1A-Thr177）浓度。

These protocols are for reference only. Biotopped does not independently validate these methods.

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