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大鼠前列腺素F1α(PGF1α)竞争法ELISA Kit

TOPEL03980
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2-8℃
6个月
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEL03980 96T $412.80
TOPEL03980 48T $275.20
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Introduction：

实验原理：

本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。在预包被抗大鼠前列腺素F1α（PGF1α）抗体（固相抗体）的微孔酶标板中，加入大鼠前列腺素F1α（PGF1α）校准品和待测样本，再加入HRP标记的大鼠前列腺素F1α（PGF1α）抗原（酶标抗原），经过温育与充分洗涤，去除未结合的组分，在微孔板固相表面形成固相抗体-酶标抗原的免疫复合物。加底物A和B，底物在HRP催化下，产生蓝色产物，在终止液（2M 硫酸）作用下，最终转化为黄色，在酶标仪450nm波长上测定吸光度（OD值），吸光度（OD值）与待测样品中大鼠前列腺素F1α（PGF1α）的浓度负相关。拟合校准品曲线，可以计算出样本中大鼠前列腺素F1α（PGF1α）的浓度。

These protocols are for reference only. Biotopped does not independently validate these methods.

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