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13-羟基十八碳三烯酸(13-HOT)竞争法ELISA Kit

TOPEL04140
2-8℃避光
6个月
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEL04140 48T $192.64
TOPEL04140 96T $261.44
Product Name
Introduction：

检测原理:

试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被13-羟基十八碳三烯酸(13-HOT)抗体的包被微孔中，依次加入标本、标准品、HRP标记的竞争抗原，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的13-羟基十八碳三烯酸(13-HOT)呈负相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。

These protocols are for reference only. Biotopped does not independently validate these methods.

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