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大鼠(Rat(沉默调节蛋白1(SIRT1)ELISA Kit

TOPEL303223
2-8℃避光
6个月
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEL303223 96T $233.92
TOPEL303223 48T $165.12
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Introduction：

试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被沉默调节蛋白1（SIRT1）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的沉默调节蛋白1（SIRT1）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。

These protocols are for reference only. Biotopped does not independently validate these methods.

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