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Western Stripping Buffer (Highly Alkaline)

Top1033
RT
1年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
Top1033 100ml $35.78
Top1033 500ml $74.30
Product Name Western Stripping Buffer (Highly Alkaline)
Introduction：

      Western抗体洗脱液(Stripping Buffer)又称抗体剥离液、膜再生液或Western一抗二抗去除液，用于Western中转移了蛋白的膜的重复利用，在Western中完成了一抗二抗结合和后续的化学发光检测后，有时还需要检测GAPDH、β-actin等表达量相对稳定的管家蛋白或其它蛋白作为参照。Stripping Buffer可以洗脱一抗二抗，非常方便地重新利用使用过的膜，检测其它蛋白，Stripping Buffer多次重复使用同一张膜，会导致蛋白信号的减弱不宜重复利用膜，一般可以利用2～3次。

      Western Stripping Buffer(强碱性)是强烈型抗体剥离液，主要依靠强碱性来解除一抗二抗的结合，可能会导致转移到膜上的蛋白部分损失，适用于Western化学发光检测后的蛋白膜的重复利用，尤其适用于PVDF膜，其次为硝酸纤维素膜，非化学发光试剂进行的Western检测(如DAB，NBT/BCIP)不适用于本试剂。该试剂仅用于科研领域，不适用于临床诊断或其他用途。

These protocols are for reference only. Biotopped does not independently validate these methods.

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