毛细管电泳条件:
Run buffer:The running buffer contained 40 mmol/L NaH2PO4, 5 mmol/L Brij 35 and 40 mmol/L H3PO4 at pH 2.2.The sample solution was 50 mmol/LHAc.
非涂层毛细管:50μml.D.,总长/有效长度:30/20cm,分离电压:11kV(电流大概46μA),进样:0.5 psi,3s,检测波长:214nm。
毛细管使用之前使用1M NaOH、0.1M NaOH、H2O各冲洗20 min。
These protocols are for reference only. Biotopped does not independently validate these methods.
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