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1M IPTG solution

Top9318S
-20℃
18个月
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
Top9318S 5ml $19.26
Product Name 1M IPTG solution
Introduction： IPTG是β–半乳糖苷酶的活性诱导物质。蓝白斑筛选中，IPTG作为安慰性诱导物诱导细菌内的蛋白表达，X-gal作为生色底物。其原理为IPTG可诱导载体Lac操纵子DNA区段合成β-半乳糖苷酶氨基端片段，该片段可与宿主细胞编码的缺陷型β-半乳糖苷酶实现基因内互补（α互补），实现α互补的细菌铺在含有X-gal生色底物的培养基上，可形成蓝色菌落；而外源DNA插入质粒的多克隆位点后，可破坏α互补作用，培养基上将长出白色菌落。IPTG也是常用的基因工程中重组蛋白表达的诱导剂。 本产品为1 M的IPTG溶液，经过过滤除菌处理，可直接加入培养基中使用。

These protocols are for reference only. Biotopped does not independently validate these methods.

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