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Yeast Hybridization Media

Yeast HighPure Plasmid Mini Kit

Catalog No.： Top9396T

Storage Condition：按说明书保存

Shelf Life： 1年

Bulk Inquiry? Manual Download Collection Product

Catalog No.	Specification	List Price
Top9396T	50T	$61.92
Top9396T	100T	$82.56
Top9396T	200T	$137.60

Product Name	Yeast HighPure Plasmid Mini Kit
Introduction：	本试剂盒采用改进SDS-碱裂解法裂解细胞并结合lytic Enzyme特异消化酵母细胞壁，能在1小时内从酵母培养液中分离出高纯度质粒DNA。酵母收集后，加入破壁酶去除细胞壁后，然后碱裂法裂解细胞，离心吸附柱内的硅基质膜在高盐、低PH值状态下选择性地结合溶液中的质粒DNA，再通过去蛋白液和漂洗液将杂质和其它细菌成分去除，最后低盐、高PH值的洗脱缓冲液将纯净质粒DNA从硅基质膜上洗脱。

Product Name

Yeast HighPure Plasmid Mini Kit

Introduction：

本试剂盒采用改进SDS-碱裂解法裂解细胞并结合lytic Enzyme特异消化酵母细胞壁，能在1小时内从酵母培养液中分离出高纯度质粒DNA。酵母收集后，加入破壁酶去除细胞壁后，然后碱裂法裂解细胞，离心吸附柱内的硅基质膜在高盐、低PH值状态下选择性地结合溶液中的质粒DNA，再通过去蛋白液和漂洗液将杂质和其它细菌成分去除，最后低盐、高PH值的洗脱缓冲液将纯净质粒DNA从硅基质膜上洗脱。

These protocols are for reference only. Biotopped does not independently validate these methods.

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