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Basic Protein Native PAGE Gel Preparation and Electrophoresis Kit

Catalog No.： Top9433T

Storage Condition：按说明书保存

Shelf Life： 1年

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
Top9433T	50次	$134.16

Product Name	Basic Protein Native PAGE Gel Preparation and Electrophoresis Kit
Introduction：	本公司提供的试剂盒包含碱性蛋白凝胶制备及电泳所需的全部试剂，用户只需自备制胶器具和蒸馏水。本试剂盒可用于配制碱性蛋白非变性(native)PAGE凝胶。本试剂盒可配制30-50块常规大小的非变性PAGE胶。各种蛋白质分子由于所含的碱性氨基酸和酸性氨基酸的数目不同，因而有各自的等电点。凡碱性氨基酸含量较多的蛋白质称为碱性蛋白质，等电点偏碱性，如组蛋白、精蛋白等。反之，凡酸性氨基酸含量较多的蛋白质，等电点就偏酸性。分离碱性蛋白时候，要利用低 pH 凝胶系统，分离酸性蛋白时候，要利用高 pH 凝胶系统。酸性蛋白通常在非变性凝胶电泳中采用的 pH 是 8.8的缓冲系统，蛋白会带负电荷，蛋白会相阳极移动；而碱性蛋白通常电泳是在微酸性环境下进行，蛋白带正电荷，这时候需要将阴极和阳极倒置才可以电泳。

Product Name

Basic Protein Native PAGE Gel Preparation and Electrophoresis Kit

Introduction：

本公司提供的试剂盒包含碱性蛋白凝胶制备及电泳所需的全部试剂，用户只需自备制胶器具和蒸馏水。本试剂盒可用于配制碱性蛋白非变性(native)PAGE凝胶。本试剂盒可配制30-50块常规大小的非变性PAGE胶。

各种蛋白质分子由于所含的碱性氨基酸和酸性氨基酸的数目不同，因而有各自的等电点。凡碱性氨基酸含量较多的蛋白质称为碱性蛋白质，等电点偏碱性，如组蛋白、精蛋白等。反之，凡酸性氨基酸含量较多的蛋白质，等电点就偏酸性。分离碱性蛋白时候，要利用低 pH 凝胶系统，分离酸性蛋白时候，要利用高 pH 凝胶系统。酸性蛋白通常在非变性凝胶电泳中采用的 pH 是 8.8的缓冲系统，蛋白会带负电荷，蛋白会相阳极移动；而碱性蛋白通常电泳是在微酸性环境下进行，蛋白带正电荷，这时候需要将阴极和阳极倒置才可以电泳。

These protocols are for reference only. Biotopped does not independently validate these methods.

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Basic Protein Native PAGE Gel Preparation and Electrophoresis Kit

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