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PEG/LiAc酵母转化液(无菌)

Catalog No.： Top9524S

Storage Condition： RT

Shelf Life： 1年

Bulk Inquiry? Manual Download Collection Product

Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
Top9524S	5ml	$23.12

Product Name
Alias	LiAc Transformation Solution
Introduction：	注意事项：本产品为酵母专用，勿反复冻融产品介绍：本产品为PEG/LiAc混合液（50% PEG3350、1 M LiAc和10×TE Buffer配制体积比为8:1:1），经过过滤除菌处理。本产品为即用型产品，无需稀释和除菌等操作，可直接用于酿酒酵母感受态细胞转化，每个转化反应需要500 µL PEG/LiAc（文库转化需2.5 mL）。 PEG在酵母转化中起到在高浓度LiAc环境中保护细胞膜，减少LiAc对细胞膜过度损伤的作用，同时PEG可促使质粒与细胞膜更紧密接触。 LiAc中Li+能够改变细胞膜的通透性，促进感受态的形成，使细胞易于吸收外源DNA。TE缓冲液能够提供稳定的pH值环境。

Product Name

Alias

LiAc Transformation Solution

Introduction：

注意事项：
本产品为酵母专用，勿反复冻融

产品介绍：
本产品为PEG/LiAc混合液（50% PEG3350、1 M LiAc和10×TE Buffer配制体积比为8:1:1），经过过滤除菌处理。

本产品为即用型产品，无需稀释和除菌等操作，可直接用于酿酒酵母感受态细胞转化，每个转化反应需要500 µL PEG/LiAc（文库转化需2.5 mL）。

PEG在酵母转化中起到在高浓度LiAc环境中保护细胞膜，减少LiAc对细胞膜过度损伤的作用，同时PEG可促使质粒与细胞膜更紧密接触。

LiAc中Li+能够改变细胞膜的通透性，促进感受态的形成，使细胞易于吸收外源DNA。TE缓冲液能够提供稳定的pH值环境。

These protocols are for reference only. Biotopped does not independently validate these methods.

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PEG/LiAc酵母转化液(无菌)

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