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1×PBS缓冲液(0.01mol/L,pH7.2)无菌

Catalog No.： Top9626S-DZ

Storage Condition： RT

Shelf Life：定制产品,货期短,到货后请尽快使用(一周内)。

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
Top9626S-DZ	500ml	$22.02

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Introduction：	样品稀释液的配制: A.1 A液 0.2mol/L磷酸二氢钠水溶液:NaH₂PO₄·H₂O 27.6g,溶于蒸馏水中，最后定容至1000mL。 A.2 B液 0.2 mol/L 磷酸氢二钠水溶液:Na2HPO₄·7H₂O 53.6 g, 或 Na₂HPO₄. 12H₂O 71.6 g或Na₂HPO₄.2H₂O 35.6g)加蒸馏水溶解，最后定容至1000mL。 A.3 pH 7.2 0.01 mol/L PBS 取A液14 mL、B液36 mL,加 NaCl 8.5g,用蒸馏水定容至1000mL。经121°C士2°C、15min高压灭菌。 A.4样品稀释液 1000 mL无菌PBS,无菌条件下分别加人青霉素2000U/mL、链霉素(2mg/mL)、庆大霉素(50ug/mL)、霉菌抑制素(1000U/mL)和牛血清白蛋白(5mg/mL)或10%终体积的甘油。如果用作粪便和泄殖腔拭子的缓冲液，抗生素可提高10倍。

Product Name

Introduction：

样品稀释液的配制:

A.1 A液

0.2mol/L磷酸二氢钠水溶液:NaH₂PO₄·H₂O 27.6g,溶于蒸馏水中，最后定容至1000mL。

A.2 B液

0.2 mol/L 磷酸氢二钠水溶液:Na2HPO₄·7H₂O 53.6 g, 或 Na₂HPO₄. 12H₂O 71.6 g或Na₂HPO₄.2H₂O 35.6g)加蒸馏水溶解，最后定容至1000mL。

A.3 pH 7.2 0.01 mol/L PBS

取A液14 mL、B液36 mL,加 NaCl 8.5g,用蒸馏水定容至1000mL。经121°C士2°C、15min高压灭菌。

A.4样品稀释液

1000 mL无菌PBS,无菌条件下分别加人青霉素2000U/mL、链霉素(2mg/mL)、庆大霉素(50ug/mL)、霉菌抑制素(1000U/mL)和牛血清白蛋白(5mg/mL)或10%终体积的甘油。如果用作粪便和泄殖腔拭子的缓冲液，抗生素可提高10倍。

These protocols are for reference only. Biotopped does not independently validate these methods.

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1×PBS缓冲液(0.01mol/L,pH7.2)无菌

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