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Agarose (Low EEO)

A6202H
RT
2年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
A6202H 250g $151.36
Product Name Agarose (Low EEO)
Introduction：        Agarose (Low EEO)，agarose with low electroendosmosis，即低电渗琼脂糖，是分子生物学级高纯度琼脂糖，不含DNase、RNase和Protease。
       本产品常用于配制核酸分析检测用凝胶，例如用于DNA或RNA电泳的琼脂糖凝胶等。常使用TAE或TBE作为电泳缓冲液。
       本产品为白色粉末，采用绿色环保的工艺生产，生产过程中不涉及有机溶剂，可有效避免有机溶剂残留对于后续实验的影
响。
本产品相关技术参数如下表：
级别   EEO   凝胶强度
(1% gel)		   凝胶温度  
(1.5% gel)		    融胶温度  
(1.5% gel)  		   水分       灰分    
分子生物学级  ≤0.13    ≥1200g/cm2 36±1.5℃ 88±1.5℃ ≤10% ≤0.5%

      本产品为低电渗，有助于和加快核酸条带电泳速度、减少条带扩散并提高电泳分辨率。并且本产品凝胶强度高，染色背景低，品质稳定。
      琼脂糖凝胶的浓度和DNA电泳的分辨率及理想的电泳液参考下表。对于分辨率要求不高时，使用TAE(Tris-乙酸EDTA缓冲液)和TBE(Tris-硼酸EDTA缓冲液)均可；对于分辨率要求比较高时，较低浓度的胶有利于提高大分子量核酸的分辨率，此时宜使用TAE；而较高浓度的胶有利于提高小分子量核酸的分辨率，此时宜使用TBE。
 
胶浓度(w/v) 理想分离范围 理想的电泳液
0.8% 800-22,000 bp TAE
1.0% 500-10,000 bp TAE/TBE
1.2% 400-7,000 bp TAE/TBE
1.5% 250-5000 bp TAE/TBE
2.0% 150-3,000 bp TBE


注意事项：
      配制琼脂糖凝胶时容易暴沸，需注意防止烫伤。    
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

These protocols are for reference only. Biotopped does not independently validate these methods.

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