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OPTI MEM I减血清培养基

DM0012D
2-8℃
1年
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
DM0012D 500ml $103.20
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Introduction:

产品主要成分:OPTI MEM I培养基含HEPES、L-谷胺酰胺及2400 mg/L碳酸氢钠,pH值为7.2-7.5。

Opti-MEM I减血清培养基是改良版Minimal Essential Medium(MEM),可使胎牛血清添加量减少至少50%,且不影响细胞生长速率或形态。该培养基还推荐与阳离子脂质体转染试剂(如Lipofectamine试剂)配套使用,适用于Sp2、AE-1、CHO、BHK-21、HEK及原代成纤维细胞等多种悬浮和贴壁哺乳动物细胞的培养。针对不同细胞培养需求,还提供多种组分改良的Opti-MEM I培养基。

Opti-MEM I培养基的使用

Opti-MEM I减血清培养基含有胰岛素、转铁蛋白、次黄嘌呤、胸苷及微量元素等独特组分,可使血清添加量减少至少50%。该培养基采用2.4 g/L碳酸氢钠缓冲体系,因此需在5–10% CO₂环境中维持生理pH值。

These protocols are for reference only. Biotopped does not independently validate these methods.

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