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1/2MM培养基(1/2YNB,用于毕赤酵母)

Catalog No.： MM2026G

Storage Condition： -20℃

Shelf Life：长期

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
MM2026G	500ml	$28.62

Product Name
Introduction：	基础甲醇培养基（Minimal Methanol Medium）即MM培养基，主要用于诱导前扩增菌体。 MM培养基加入组氨酸，即为MMH培养基，用于毕赤酵母菌甲醇诱导的细胞内蛋白表达。尤其适用于有蛋白酶活性的重组蛋白的表达。 MMMa培养基(Minimal Methanol and Methylamine Medium)即基础甲醇甲胺培养基，用于以Zeocin为筛选标记，以甲醇和甲胺为诱导剂的毕赤酵母重组蛋白表达系统：pFLD载体、pFLDα载体、X-33酵母、KM71H酵母、SMD1168H酵母。 MM这一组培养基的特点是缺少缓冲剂，属于成分明确的培养基。适用于GS115、KM71、X33、SMD1163、SMD1168等毕赤酵母菌株。 MM培养基用于蛋白表达小试阶段，而MMH培养基则用于蛋白表达的放大阶段。

Product Name

Introduction：

基础甲醇培养基（Minimal Methanol Medium）即MM培养基，主要用于诱导前扩增菌体。

MM培养基加入组氨酸，即为MMH培养基，用于毕赤酵母菌甲醇诱导的细胞内蛋白表达。

尤其适用于有蛋白酶活性的重组蛋白的表达。

MMMa培养基(Minimal Methanol and Methylamine Medium)即基础甲醇甲胺培养基，用于以Zeocin为筛选标记，

以甲醇和甲胺为诱导剂的毕赤酵母重组蛋白表达系统：pFLD载体、pFLDα载体、X-33酵母、KM71H酵母、SMD1168H酵母。

MM这一组培养基的特点是缺少缓冲剂，属于成分明确的培养基。

适用于GS115、KM71、X33、SMD1163、SMD1168等毕赤酵母菌株。

MM培养基用于蛋白表达小试阶段，而MMH培养基则用于蛋白表达的放大阶段。

These protocols are for reference only. Biotopped does not independently validate these methods.

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1/2MM培养基(1/2YNB,用于毕赤酵母)

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