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polyphenol oxidase，PPO

TOPEK190895
4℃
6个月
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEK190895 50T/24S $74.30
Product Name polyphenol oxidase，PPO
Introduction： 多酚氧化酶（PPO）活性检测试剂盒说明书
注意：正式测定前务必取2-3个预期差异较大的样本做预测定
货号：TOPEK190895
规格：50管/24样

产品简介：

PPO 主要存在于动物、植物、微生物和培养细胞中，是一种含铜的氧化酶，能使一元酚和二元酚氧化产生醌，从而引起褐化，与果蔬加工、茶叶品质和组培等密切相关。
PPO 能够催化邻苯二酚产生醌，后者在 410nm 有特征光吸收。

试验中所需的仪器和试剂：

可见分光光度计、台式离心机、水浴锅、可调式移液器、1mL 玻璃比色皿、研钵、冰和蒸馏水。

产品内容：

提取液：液体 60mL×1 瓶，4℃保存，用前混匀； 试剂一：液体 40mL×1 瓶，4℃保存；
试剂二：液体 10mL×1 瓶，4℃保存。

操作步骤：

一、粗酶液提取：
  1. 收集细菌或细胞到离心管内，离心后弃上清；按照每 500 万细菌或细胞加入 1mL 提取液，超声波破碎细菌或细胞（功率 20％，超声 3 秒，间隔 10 秒，重复 30 次）；8000g 4℃离心 10 分钟，取上清，置冰上待测。
  2. 称取约 0.1g 组织，加入 1mL 提取液进行冰浴匀浆。8000g 4℃离心 10 分钟，取上清，置冰上待测。

二、测定操作表：

1、分光光度计预热 30min 以上，调节波长至 410nm，蒸馏水调零。
2、样本测定（在 EP 管中依次加入下列试剂）



 

These protocols are for reference only. Biotopped does not independently validate these methods.

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