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α-葡萄糖苷酶抑制剂检测试剂盒(PNPG法)(分光光度法)

TOPEK191202
4℃/-20℃
6个月
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TOPEK191202 100T/48S $128.79
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Introduction：

正式测定前务必取 2-3 个预期差异较大的样本做预测定

产品介绍：

本试剂盒专为α-葡萄糖苷酶抑制剂的体外筛选与活性检测而设计。α-葡萄糖苷酶是一种重要的消化酶，能够催化糖苷键的水解。本试剂盒以对硝基苯基-α-D-吡喃葡萄糖苷 (PNPG) 作为底物，通过其水解产物对硝基苯酚的显色反应，能够快速、准确地测定α-葡萄糖苷酶的活性及其抑制剂的作用效果。

通过有效抑制α-葡萄糖苷酶的活性，可以延缓或降低糖类物质的水解与吸收，使其成为开发功能性食品和药物的重要靶点。本试剂盒提供了一种简单、高通量、可靠的检测方法，是您进行相关科学研究与产品研发的理想工具。

测定原理：

本试剂盒采用对硝基苯酚比色法，通过检测α-葡萄糖苷酶作用下产生的有色产物来评估酶的活性。在碱性条件下，反应产物对硝基苯酚会迅速转化为黄色的对硝基苯酚盐。该黄色产物的吸光度在400 nm波长处具有特征吸收，其吸光值与体系中产生的对硝基苯酚量成正比。

These protocols are for reference only. Biotopped does not independently validate these methods.

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