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活性氧检测试剂盒(红色荧光)

Catalog No.： TOPEK191206

Storage Condition： -20℃

Shelf Life： 6个月

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
TOPEK191206	100-500T	$147.23
TOPEK191206	200T	$74.30

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Introduction：	测定意义：活性氧（Reactive Oxygen Species，ROS）是生物体内与氧代谢相关的含氧自由基和易形成自由基的过氧化物的总称，包括超氧阴离子、过氧化氢、羟自由基、烷氧基、烷过氧基等。其与机体氧化损伤密切相关，参与人体多种生理和病理过程，过量产生会损伤细胞组分。测定原理：利用荧光探针DHE进行活性氧检测是一种基于荧光强度变化，检测细胞内活性氧水平的经典方法，DHE可以自由穿过细胞膜，进入细胞内后，在细胞质中呈蓝色荧光，被细胞内的ROS氧化后主要生成 2-羟基乙锭（超氧阴离子特异性产物）和乙锭，二者均可掺入染色体DNA中，使细胞核呈现明亮红色荧光，最大激发波长为488~535nm（最适激发波长是518nm），最大发射波长为610nm，使用荧光显微镜检测荧光信号，测定细胞内活性氧水平。

Product Name

Introduction：

测定意义：

活性氧（Reactive Oxygen Species，ROS）是生物体内与氧代谢相关的含氧自由基和易形成自由基的过氧化物的总称，包括超氧阴离子、过氧化氢、羟自由基、烷氧基、烷过氧基等。其与机体氧化损伤密切相关，参与人体多种生理和病理过程，过量产生会损伤细胞组分。

测定原理：

利用荧光探针DHE进行活性氧检测是一种基于荧光强度变化，检测细胞内活性氧水平的经典方法，DHE可以自由穿过细胞膜，进入细胞内后，在细胞质中呈蓝色荧光，被细胞内的ROS氧化后主要生成 2-羟基乙锭（超氧阴离子特异性产物）和乙锭，二者均可掺入染色体DNA中，使细胞核呈现明亮红色荧光，最大激发波长为488~535nm（最适激发波长是518nm），最大发射波长为610nm，使用荧光显微镜检测荧光信号，测定细胞内活性氧水平。

These protocols are for reference only. Biotopped does not independently validate these methods.

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活性氧检测试剂盒(红色荧光)

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