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Glutamine Synthetase (GS) Assay Kit (Micro Method)​

TOPEK190909
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TOPEK190909 100T/48S $82.56
Product Name Glutamine Synthetase (GS) Assay Kit (Micro Method)​
Introduction：

正式测定前务必取2-3个预期差异较大的样本做预测定                                             

测定意义：

GS（EC 6.3.1.2）主要存在于植物中，是生物体内氨同化的关键酶之一，催化铵离子和谷氨酸合成谷氨酰胺，不仅可以防止过多的铵离子对生物有毒性，而且谷氨酰胺也是氨的主要储存和运输形式。

测定原理：

GS在ATP和Mg2+存在下，催化铵离子和谷氨酸合成谷氨酰胺；谷氨酰胺进一步转化为γ─谷氨酰基异羟肟酸，在酸性条件下形成的络合物在540nm处有最大吸收峰，可用分光光度计测定。

These protocols are for reference only. Biotopped does not independently validate these methods.

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