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Glutamine Synthetase (GS) Assay Kit (Micro Method)​

TOPEK190909
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6个月
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TOPEK190909 100T/48S $82.56
Product Name Glutamine Synthetase (GS) Assay Kit (Micro Method)​
Introduction：

正式测定前务必取2-3个预期差异较大的样本做预测定

测定意义：

谷氨酰胺合成酶（GS）作为高等植物氮代谢中的关键酶，能够催化铵离子和谷氨酸合成谷氨酰胺参与氨同化过程，不仅可以防止铵离子过多造成的生物毒性，而且谷氨酰胺也是氨的主要储存和运输形式，谷氨酰胺合成酶活性可作为衡量植物氮素同化水平的一项重要生理指标。

测定原理：

谷氨酰胺合成酶在ATP和 Mg2+存在下，催化羟胺和谷氨酸合成γ-谷氨酰基异羟肟酸，在酸性条件下与铁形成红色的络合物，产物在 540 nm 处具有特征吸收峰，通过吸光值的变化即可表征GS的活性。

These protocols are for reference only. Biotopped does not independently validate these methods.

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