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SYT0 Green Fluorescent Nucleic Acid Stain

HS003
2-8℃避光干燥
1年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
HS003 25μl $96.04
HS003 250μl $508.84
Product Name SYT0 Green Fluorescent Nucleic Acid Stain
Appearance (character) 橙色溶液
Introduction：

SYTOX Green 核酸染料是一种极佳的绿色荧光核和染色体复染剂，不可透过活细胞，从而可作为细胞群中死细胞的有效指示剂。

SYTOX Green 可使用流式细胞仪、荧光显微镜、荧光计或荧光微孔板酶标仪快速测定细胞活力。其不会穿过完整的细胞膜，但可轻易穿透受损的细胞膜，而这正是死细胞的特征。由于结合核酸后，荧光会增强 >500 倍，所以使用 SYTOX Green 与死细胞短暂孵育后，在通过 488 nm 氩离子激光或任何其他 450–490 nm 的光源激发时，会产生明亮的绿色荧光，发射峰为 523 nm。死细胞产生的信号非常明亮，因此 SYTOX Green 不仅特别适用于确定哺乳动物细胞的活力，还可用于革兰氏阳性和革兰氏阴性细菌的活力测定。

本品以DMSO 储存液形式提供，浓度为 5mM。只需用合适的生理缓冲液稀释到工作浓度进行简单孵育即可。适用于哺乳动物细胞、和革兰氏阴性菌。若需做双染,可直接购买我司的 SYTOX/PILive/Dead Bacterial Double Stain Kit 活细菌/死细菌双染试剂盒。

These protocols are for reference only. Biotopped does not independently validate these methods.

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