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Ca++Mg++-ATP酶检测试剂盒(微量法)

TOPEK190765
按说明书保存
6个月
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TOPEK190765 100T/48S $63.30
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Introduction:

正式测定前务必取2-3个预期差异较大的样本做预测定

测定意义Ca++ Mg++-ATP 酶广泛分布于植物、动物、微生物和细胞中,可催化ATP水解生成ADP和无机磷。

测定原理:根据Ca++ Mg++ -ATP 酶分解ATP生成ADP及无机磷,通过测定无机磷的量来确定ATP酶活性高低。

需自备的仪器及用品:可见分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。

试剂的组成和配制:

提取液:液体 100mL×1 瓶,4℃保存。

试剂一:液体 10mL×1 瓶,4℃保存。

试剂二:粉剂×1 瓶,-20℃保存;临用前加入6mL蒸馏水充分混匀待用;用不完的试剂 4℃保存一周;

试剂三:液体 2mL×1 瓶,4℃保存。

试剂四:粉剂×1 瓶,4℃保存。用时加入3mL蒸馏水, 4℃保存。

试剂五:粉剂×1 瓶, 4℃保存。用时加入25mL蒸馏水,溶解后 4℃保存一周。

试剂六:粉剂×1 瓶, 4℃保存。用时加入25mL蒸馏水,溶解后 4℃保存一周。

试剂七:液体25mL×1 瓶,室温保存。

试剂八:10mmol/L 标准磷贮备液 10mL×1 瓶,4℃保存。

0.5μmol/mL标准磷应用液配制:将试剂八20倍稀释,即取0.1mL试剂八加1.9mL蒸馏水充分混匀。

定磷剂的配制:按 H2O: 试剂五:试剂六:试剂七=2:1:1:1 的比例配制,配好的定磷剂应为浅黄色。若无色则试剂失效,若是蓝色则为磷污染,定磷剂现用现配。

注意:配试剂最好用新的烧杯、玻棒和玻璃移液器,也可以用一次性塑料器皿,避免磷污染。

These protocols are for reference only. Biotopped does not independently validate these methods.

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