Domestic Area
Welcome to Biotopped Please log in Sign in
CN EN
Potassium chloride Ethylene glycol iodomethane Cuprous bromide
0 Shopping Cart
010-59412420/30
Your current location：
  1. Home
  2. All Product Categories
  3. Reagent products
  4. Protein and Immunology Products
  5. Biochemical Assay Kits
Product Catalog

Ca++Mg++-ATP酶检测试剂盒(微量法)

TOPEK190765
按说明书保存
6个月
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TOPEK190765 100T/48S $72.24
Product Name
Introduction：

正式测定前务必取2-3个预期差异较大的样本做预测定

测定意义：Ca++ Mg++-ATP 酶广泛分布于植物、动物、微生物和细胞中，可催化ATP水解生成ADP和无机磷。

测定原理：根据Ca++ Mg++ -ATP 酶分解ATP生成ADP及无机磷，通过测定无机磷的量来确定ATP酶活性高低。

需自备的仪器及用品:可见分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。

试剂的组成和配制：

提取液：液体 100mL×1 瓶，4℃保存。

试剂一：液体 10mL×1 瓶，4℃保存。

试剂二：粉剂×1 瓶，-20℃保存；临用前加入6mL蒸馏水充分混匀待用；用不完的试剂 4℃保存一周；

试剂三：液体 2mL×1 瓶，4℃保存。

试剂四：粉剂×1 瓶，4℃保存。用时加入3mL蒸馏水， 4℃保存。

试剂五：粉剂×1 瓶, 4℃保存。用时加入25mL蒸馏水，溶解后 4℃保存一周。

试剂六：粉剂×1 瓶, 4℃保存。用时加入25mL蒸馏水，溶解后 4℃保存一周。

试剂七：液体25mL×1 瓶，室温保存。

试剂八：10mmol/L 标准磷贮备液 10mL×1 瓶，4℃保存。

0.5μmol/mL标准磷应用液配制：将试剂八20倍稀释，即取0.1mL试剂八加1.9mL蒸馏水充分混匀。

定磷剂的配制：按 H2O: 试剂五:试剂六:试剂七=2:1:1:1 的比例配制，配好的定磷剂应为浅黄色。若无色则试剂失效，若是蓝色则为磷污染，定磷剂现用现配。

注意：配试剂最好用新的烧杯、玻棒和玻璃移液器，也可以用一次性塑料器皿，避免磷污染。

These protocols are for reference only. Biotopped does not independently validate these methods.

No specification！
Search quality inspection report(COA)
No technical Q&A content!

Related Products

Solid-Nitrate Reductase
TOPEK191164 24T
$130.72
Solid-Nitrate Reductase
TOPEK191163 48T
$233.92
Nitrate Reductase
TOPEK191162 100T/96S
$94.94
Nitrate Reductase
TOPEK191161 24T
$68.80
TOPEK190976 100T
$151.36
TOPEK191057 100T
$132.10
TOPEK190750 50T
$49.54
TOPEK190980 100T/96S
$344.00
TOPEK191160 96样
$82.56
TOPEK191159 96样
$90.82
TOPEK190845 96样
$93.57
TOPEK190847 48样
$46.78
TOPEK190506 96样
$82.56
TOPEK190754 96样
$79.81
TOPEK190568 48样
$79.81
TOPEK190755 100T/96S
$121.09
Aspartate Aminotransferase (AST) and Glutamic-Oxaloacetic Transaminase (GOT) Assay Kit (Visible Spectrophotometric Method)
TOPEK190514 50T/24S
$28.90
Aspartate Aminotransferase (AST) Assay Kit (Micromethod)
TOPEK190521 100T/48S
$44.03
Alanine Aminotransferase (ALT) and Glutamic-Pyruvic Transaminase (GPT) Assay Kit (UV-Vis Spectrophotometric Method)
TOPEK190519 50T/24S
$27.52
Alanine Aminotransferase (ALT) Assay Kit (Micromethod)
TOPEK190532 100T/48S
$41.28

HeadquartersBeijing

Enquiry Hotline 010-5941-2420
Enquiry Hotline 010-5941-2430
Wechat

All products sold on this website are only used for industrial applications or scientific research and other non-medical purposes, and cannot be used for clinical diagnosis or treatment of humans or animals, non-medicinal, non-edible.

 © Beijing Biotopped 2017-2023 , All Rights Reserved 

 Technical Support：Kuujia
Home Sort Shopping Cart Member