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DAF-FM DA(NO Fluorescence Probe)

TOPEK191200
-20℃避光
1年
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TOPEK191200 100T $178.88
Product Name DAF-FM DA(NO Fluorescence Probe)
Introduction：

     DAF-FM DA即3-Amino,4-aminomethyl-2',7'-difluorescein, diacetate ，也称DAF-FM diacetate或4-Amino, 5-aminomethyl-2', 7'- difluorescein, diacetate。DAF-FM DA是最新一代用于检测一氧化氮荧光探针，比以前比较常用的一氧化氮荧光检测探针DAF-2 diacetate有多方面的改进。首先，DAF-FM DA和DAF-2 diacetate相比，最后和一氧化氮反应形成的荧光产物受pH  值的影响小，在pH值大于5.5时不受pH值的影响。其次，DAF-FM DA和DAF-2 diacetate相比，前者产生的荧光更加稳定，不容易淬灭，这样更加便于检测。另外，DAF-FM DA和DAF-2 diacetate相比，前者对一氧化氮的检测灵敏度更高，相同条件下检测灵敏度可以提高接近2倍，最低检测浓度可以达到3nM。

      DAF-FM DA可以穿过细胞膜(cell-permeable) ，进入细胞后可以被细胞内的酯酶催化形成不能穿过细胞膜的DAF-FM  。DAF-FM本身仅有很弱的荧光，但在和一氧化氮反应后可以产生强烈荧光，激发波长为495nm ，发射波长为515nm 。DAF-FM DA检测一氧化氮的机制可以参考上图。任何可以检测fluorescein的仪器，包括荧光显微镜、激光共聚焦显微镜、流式细胞仪、荧光分光光度计或荧光酶标仪都可以用于该荧光探针的检测。下图为DAF-FM在不同浓度一氧化氮存在时的发射荧光扫描图谱(fluorescence emission spectra)。

These protocols are for reference only. Biotopped does not independently validate these methods.

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