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Na +K+ - ATP 酶活性测定试剂盒(微量法)

Catalog No.： TOPEK190767

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Shelf Life： 6个月

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
TOPEK190767	100T/48S	$60.54

Product Name
Introduction：	产品简介： Na⁺K⁺- ATP酶广泛分布于植物、动物、微生物和细胞中，可催化ATP水解生成ADP和无机磷。Na⁺K⁺-ATP酶分解ATP生成ADP及无机磷，通过测定无机磷的量来确定ATP酶活性。自备的仪器和用品：酶标仪/可见分光光度计、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。产品内容：提取液：液体100mL×1 瓶，4℃保存。试剂一：液体 10mL×1 瓶，4℃保存。试剂二：粉剂×1瓶，-20℃保存；临用前加入6ml蒸馏水充分混匀待用；用不完的4℃保存一周。试剂三：液体 2ml×1 瓶，4℃保存。试剂四：粉剂×1瓶，4℃保存。用时加入3mL蒸馏水4℃保存。试剂五：粉剂×1 瓶，4℃保存。用时加入25ml蒸馏水，溶解后4℃保存一周。试剂六：粉剂×1 瓶，4℃保存。用时加入25ml蒸馏水，溶解后4℃保存一周。试剂七：液体25ml×1瓶，室温保存。试剂八：10mmol/L 标准磷贮备液 1mL×1 瓶，4℃保存。 0.5μmol/mL 标准磷应用液配制：将试剂八 20倍稀释，即取 0.1mL试剂八加1.9mL蒸馏水充分混匀。定磷剂的配制：按H2O:试剂五:试剂六:试剂七=2:1:1:1 的比例配制，配好的定磷剂应为浅黄色。若无色则试剂失效，若是蓝色则为磷污染，定磷剂现用现配。注意：配试剂最好用新的烧杯、玻棒和玻璃移液器，也可以用一次性塑料器皿，避免磷污染。

Product Name

Introduction：

产品简介：

Na⁺K⁺- ATP酶广泛分布于植物、动物、微生物和细胞中，可催化ATP水解生成ADP和无机磷。Na⁺K⁺-ATP酶分解ATP生成ADP及无机磷，通过测定无机磷的量来确定ATP酶活性。

自备的仪器和用品：

酶标仪/可见分光光度计、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。

产品内容：

提取液：液体100mL×1 瓶，4℃保存。

试剂一：液体 10mL×1 瓶，4℃保存。

试剂二：粉剂×1瓶，-20℃保存；临用前加入6ml蒸馏水充分混匀待用；用不完的4℃保存一周。

试剂三：液体 2ml×1 瓶，4℃保存。

试剂四：粉剂×1瓶，4℃保存。用时加入3mL蒸馏水4℃保存。

试剂五：粉剂×1 瓶，4℃保存。用时加入25ml蒸馏水，溶解后4℃保存一周。

试剂六：粉剂×1 瓶，4℃保存。用时加入25ml蒸馏水，溶解后4℃保存一周。

试剂七：液体25ml×1瓶，室温保存。

试剂八：10mmol/L 标准磷贮备液 1mL×1 瓶，4℃保存。

0.5μmol/mL 标准磷应用液配制：将试剂八 20倍稀释，即取 0.1mL试剂八加1.9mL蒸馏水充分混匀。

定磷剂的配制：按H2O:试剂五:试剂六:试剂七=2:1:1:1 的比例配制，配好的定磷剂应为浅黄色。若无色则试剂失效，若是蓝色则为磷污染，定磷剂现用现配。

注意：配试剂最好用新的烧杯、玻棒和玻璃移液器，也可以用一次性塑料器皿，避免磷污染。

These protocols are for reference only. Biotopped does not independently validate these methods.

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Na +K+ - ATP 酶活性测定试剂盒(微量法)

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