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5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside ，≥99%

X6050D
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7240-90-6
-20℃
3年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
X6050D 100mg $8.26
X6050D 250mg $17.89
X6050D 1g $44.03
X6050D 5g $206.40
X6050D 10g $316.48
Product Name 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside
CAS No. 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight 408.64
Rank Ultra Pure Grade
Solubility 20mg/ml DMF
Appearance (character) 白色粉末
Purity： ≥99%
Introduction：

用于基因工程实验生化研究试剂。

作为β-半乳糖苷酶的发色底物用于蓝白斑重组细菌的筛选实验。

溶解性：溶于DMF，参考浓度20mg/ml。

 

X-gal溶解配方：常用溶剂与储存液配制
1. 溶剂选择 ：
X-gal难溶于水，易溶于二甲基甲酰胺（DMF）或二甲基亚砜（DMSO）。
推荐配方：
X-gal储存液：20 mg/mL（或40 mg/mL）溶于DMF或DMSO，避光分装保存于-20℃（避免反复冻融）。
2. 工作液配制 （以蓝白斑筛选为例）：
LB/琼脂平板 ：每100 mL培养基中加入：200 μL X-gal储存液（20 mg/mL，终浓度40 μg/mL）20 μL IPTG（100 mM，终浓度20μM）
细胞染色（如SA-β-gal检测衰老细胞） ：
终浓度通常为1 mg/mL X-gal（溶于染色缓冲液）。
X-gal的常见应用
1. 蓝白斑筛选（克隆筛选） 原理：
载体携带LacZ基因（编码β-半乳糖苷酶），在IPTG诱导下表达。 若外源片段插入导致LacZ失活，菌落呈白色（无酶活性）；未插入则呈蓝色（X-gal被水解生成蓝色产物）。

备注：产品信息可能会有优化升级。请以实际标签信息为准。

These protocols are for reference only. Biotopped does not independently validate these methods.

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