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Benzonase

M0028C
9025-65-4
-20℃
2年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
M0028C 25KU $148.61
Product Name Benzonase
CAS No. 9025-65-4
Solubility 溶解在缓冲液20mM Tris-HCl（pH 8.0），2mM MgCl22 ，20mM NaCl中。
Appearance (character) 无菌液体
Introduction：

来源：粘质沙氏菌（Serratia marcescens）

产品简介：

本产品是经过蛋白质工程技术改造的源于粘质沙氏菌（Serratiamarcescens，又称灵杆菌）的非特异性核酸内切酶。它不含有原核生物表达的细菌内毒素。在功能上，它能够剪切各种形式DNA和RNA，无论是单链、双链、线状、环状或超螺旋形式的DNA和RNA，切断磷酸二酯键从而产生5’-磷酸核苷酸或5’ 磷酸寡核苷酸。广谱核酸酶对核酸碱基序列无特异性要求，可在链内任意核苷酸间进行切割，可用于除去生物制品中的核酸。在疫苗工业、蛋白和多糖类制药工业中，广泛用于去除宿主核酸残留，可使疫苗和蛋白类产品核酸污染达到皮克（pg）级别。广谱核酸酶可以通过降解核酸来降低细胞裂解液的粘度（viscosity），有助于在病毒、AAV载体及包涵体等细胞来源颗粒 的纯化过程中促进溶液过滤/超滤过程，缩短处理时间、提高离心过程中沉淀与上清的分离效率并且提高色谱纯化效率，最终达到提高得率并且有效提高终产物纯度的效果。生物分析应用：可用于ELISA样品制备，色谱层析活双相电泳（蛋白质图谱）及印迹分析等过程中来提高分辨率和样品回收率。 

注意事项：打开包装使用后，如果在2-8ºC环境下放置超过1周时间，建议过滤除菌，防止微生物污染。

These protocols are for reference only. Biotopped does not independently validate these methods.

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