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EC-MUG Medium

P40030D
2-8℃
3年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
P40030D 250g $123.84
Product Name EC-MUG Medium
Rank BR
Introduction：

产品介绍：

EC-MUG培养基是青岛海博公司改良的培养基，用于生活饮用水及其水源水中的大肠埃希氏菌的检测。

检验原理：

胰蛋白胨提供碳氮源；3 号胆盐抑制革兰氏阳性菌，特别抑制革兰氏阳性杆菌和粪链球菌；乳糖是可发酵的糖类；磷酸氢二钾和磷酸二氢钾为缓冲剂；氯化钠可维持均衡的渗透压。大肠埃希氏菌含有的葡萄糖醛酸苷酶作用于4-甲基伞形酮-β-D 葡萄糖醛酸苷（4-Methylumbellifery-β-D-Glucuronide 简称MUG）的β糖醛酸苷键，使其水解，释放的4-甲基伞形酮在366nm 紫外灯下产生蓝白色荧光。97%的大肠埃希氏菌、10%的沙门氏菌以及少量的志贺氏菌具有葡萄糖醛酸苷酶。

用途:

用于生活饮用水及其水源水中的大肠埃希氏菌的测定

成分(g/L):

胰蛋白胨：20.0

乳糖：5.0

三号胆盐：1.5

磷酸氢二钾：4.0

磷酸二氢钾：1.5

氯化钠：5.0

MUG：0.05

pH值6.9±0.2：25℃

用法：称取本品37.0g,加热搅拌溶解于1000ml蒸馏水中115℃高压灭菌20分钟,备用。

These protocols are for reference only. Biotopped does not independently validate these methods.

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