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Collagenase IA

C6180C
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9001-12-1
-20℃
3年
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C6180C 25mg $110.08
Product Name Collagenase IA
CAS No. 9001-12-1
Molecular Weight 68-130 kDa
Rank BR
PH 6.3-8.5
Appearance (character) 棕褐色或淡褐色粉末
Introduction： 来源：溶组织棱状芽胞杆菌。
活力定义：
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions。One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37℃。One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25℃ in the presence of DTT。
不同类型胶原酶介绍：
1、胶原酶Ⅰ有着初始平衡的胶原酶、酪蛋白酶、梭菌蛋白酶和胰蛋白酶的活性，用于上皮、肺，脂肪和肾上腺组织细胞的分离。
2、胶原酶Ⅱ含有更高的梭菌蛋白酶活性，适用于肝脏，骨，甲状腺，心脏和唾液腺组织。
3、胶原酶Ⅲ次级蛋白酶的含量最低但是含有胰蛋白酶活性，用于原代乳腺细胞分离。
4、胶原酶Ⅳ含有低水平的胰蛋白酶活性以限制膜蛋白和受体的损伤，但是含有正常水平的胶原酶的活性成分，能消化多种组织,对哺乳动物的组织有广谱的消化作用，用于胰岛原代细胞分离。
5、胶原酶Ⅴ部分纯化，含有较高的胶原酶和酪蛋白酶活性。可用于胰腺小岛组织的分离，结缔组织分离。
用途：
具有独特的消化天然胶原和变性胶原能力。EDTA、DTT、六氯环己烷和重金属离子可降低酶活性。专一作用于原胶原，使其断裂进而被其他蛋白酶水解，不水解纤维蛋白，球蛋白

These protocols are for reference only. Biotopped does not independently validate these methods.

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