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Trypsin ，≥95%(HPLC)

T6153C
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9002-07-7
2-8℃
2年
Bulk Inquiry? Manual Download Collection Product
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
T6153C 100ug $330.24
T6153C 1mg $2270.40
Product Name Trypsin
CAS No. 9002-07-7
Molecular Weight 24.0±2.4kDa（电泳）
Appearance (character) 白色、类白色粉末
Purity： ≥95%(HPLC)
Introduction：

产品介绍：

酶切位点：胰蛋白酶是一种丝氨酸蛋白酶，可特异切割赖氨酸（Lysine、K）以及精氨酸（Arginine、R） C末端肽键。

氨基酸序列：重组猪胰蛋白酶是由重组大肠杆菌表达生产，氨基酸序列与猪胰腺来源的阳离子型胰蛋白酶完全一致。

不自切：胰蛋白酶容易自切产生自身自切片段影响质谱分析结果，甲基化修饰和突变保证其不自切，从而也去除了因自切而产生的假糜蛋白酶的活性。

无杂酶：无糜蛋白酶等杂酶污染，不含有且不需要TPCK抑制其他酶活性，活性高，酶切特异性高。

用途：建议使用50mMHAc或1mM HCl溶解或稀释。使用时，稀释在50mM NH4HCO3 或者ph7.0-8.0的缓冲溶液中。在酶切缓冲液中建议使用1mM CaCl2，重组胰蛋白酶：目的蛋白=1：20-1：100，最适PH为7.0-8.0。

These protocols are for reference only. Biotopped does not independently validate these methods.

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