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dntps Mix(2.5mM each)

DT535
-20℃
2年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
DT535 1ml $9.63
Product Name
Introduction： dNTPs Mix(2.5mM each)说明书
货号：DT535
规格：0.5mL/1mL
级别：PCR级别
英文名称：dNTPs Mix(2.5mM each)
外观（性状）：液体
保存条件：-20度保存 ，有效期至少2年。
产品说明：
本产品仅用于科研，不可用于其他。
2.5mM dNTP（2’-脱氧核苷酸-5’-三磷酸）Mix 包含四种脱氧核苷酸（dATP、dCTP、dGTP、dTTP），溶于0.6mM Tris-HCl（pH 7.5）中，每种核苷酸浓度均为 2.5 mM。2.5 mM dNTP Mix 可用于聚合酶链式反应（PCR）、测序、填补、切口平移、cDNA 合成及 TdT 加尾反应。
20μL 反应体系
dNTP 终浓度 dNTP 加入量 使用次数
0.5mL 1mL
0.2mM 1.6μL 312 625
0.5 mM 4.0μL 125 250
1.0 mM 8.0μL 62 125
1.5 mM 12.0μL 41 82
 
50μL 反应体系
dNTP终浓度 dNTP加入量 使用次数
0.5mL 1mL
0.2mM 4.0μL 125 250
0.5 mM 10.0μL 50 100
1.0 mM 20.0μL 25 50
1.5 mM 30.0μL 16 32
 
 
 

These protocols are for reference only. Biotopped does not independently validate these methods.

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