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TB培养基(极品肉汤)

TB5030D
RT
3年
Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
TB5030D 250g $71.55
Product Name
Introduction:

TB培养基(极品肉汤)
储存:RT 避光干燥,有效期3年
产品说明:
Terrific Broth又称TB培养基,可用于多种细菌培养,是分子生物学常规试剂。
TB培养基主要由胰蛋白胨、酵母提取物、磷酸盐等组成,其胰蛋白胨和酵母提取物的浓度比常规LB培养基高。主要用于基因工程菌大肠杆菌培养,营养丰富。
操作步骤:
称取本品 47.6g,加热溶解于1000ml蒸馏水中,每升添加4mL甘油,加热煮沸溶解,分装,121℃ 高压灭菌15分钟,备用。
成分:(g/L) 
胰酪蛋白胨                  12
酵母浸粉                    24
磷酸二氢钾                  2.2
磷酸氢二钾                  9.4
PH7.2±0.2(25℃)
注意事项:
1、注意无菌操作,尽量避免污染。
2、为了您的安全和健康,请穿实验服并带一次性手套操作。
 

These protocols are for reference only. Biotopped does not independently validate these methods.

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