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甘油三酯(TG)检测试剂盒(GPO-PAP双试剂比色法)

TOPEK190976
4℃避光
6个月
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TOPEK190976 100T $151.36
Product Name
Introduction： 用途：
用于人或动物的血清、血浆、脑脊液、细胞、组织等样本中的甘油三脂含量定量测定。
产品介绍：
甘油三酯(Triglyceride，TG)又称三酰甘油，是3分子长链脂肪酸和甘油形成的脂肪分子，是人体内含量最多的脂类，大部分组织均可以利用甘油三酯分解产物供给能量，同时肝脏、脂肪等组织还可以进行甘油三酯的合成，用酶学方法测定TG是生化检测中的常用方法，其特点是：1、灵敏度、准确度、精密度均高；2、使用温和的反应条件；3、操作简便；4、适用于半自动生化分析仪。 甘油三酯(TG)检测试剂盒(GPO-PAP双试剂比色法)又称磷酸甘油氧化酶法或磷酸甘油氧化酶-过氧化物酶偶联法等，其原理是甘油三酯被脂蛋白酯酶(LPL)水解为甘油和脂肪酸，甘油经甘油激酶(GK)和三磷酸腺苷(ATP)磷酸化为3-磷酸甘油(G-3-P)，后被磷酸甘油氧化酶(GPO)氧化并产生过氧化氢，再经过氧化物酶(POD)、4-氨基安替比林(4-AAP)与酚(三者合称PAP)反应，生成红色苯醌亚胺(Trinder反应)，苯醌亚胺的最大吸收在510nm波长处，吸光度与样本中甘油三酯含量呈正比，可通过分光光度计在500~520nm处进行比色测定。本试剂盒用于人或动物的血清、细胞、组织等样本中的甘油三酯含量定量测定。本试剂盒仅用于科研领域，不宜用于临床诊断或其他用途。

These protocols are for reference only. Biotopped does not independently validate these methods.

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