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Mannitol-Egg-Yolk-Polymyxin Agar Base

CM0056D
RT避光干燥
3年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
CM0056D 250g $38.25
Product Name Mannitol-Egg-Yolk-Polymyxin Agar Base
Introduction：

用途：用于蜡样芽孢杆菌的固体平板计数。

添加剂：

每瓶需配套添加11盒多粘菌素B(1万单位)和6盒50%卵黄乳液使用，每100ml甘露醇卵黄多粘菌素琼脂基础添加1支多粘菌素B(1万单位)和1支50%卵黄乳液。

成分(g/L):

蛋白胨 :10.0

甘露醇:10.0

牛肉浸粉:1.0

氯化钠:10.0

酚红:0.025

琼脂:15.0

pH值7.2±0.1:25℃

检验原理：

蛋白胨、牛肉浸粉在培养基中作为营养物质提供菌体生长所需的碳源、氮源、维生素及矿质元素等；甘露醇为碳水化合物及可发酵的碳源；酚红为酸碱指示剂；琼脂作为凝固剂；50%卵黄提供卵磷脂；多粘菌素B 抑制其他菌的生长。发酵甘露醇的细菌在该培养基上生长产酸使菌落及周围的培养基变成黄色，产卵磷脂酶的细菌则水解卵磷脂，使其菌落周围形成一个白色的沉淀环。蜡样芽孢杆菌为甘露醇阴性和卵磷脂酶阳性，因此其菌落呈粉红色，周围有白色的沉淀环。

用法：

称取本品46.0g，加热溶解于1000ml蒸馏水中，分装每瓶100ml，经121℃高压灭菌15分钟，待培养基冷却至50℃时，每瓶加入50%卵黄乳液5ml及多粘菌素B 10000IU，摇匀，倾入无菌平皿。

These protocols are for reference only. Biotopped does not independently validate these methods.

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