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Bacilus Cereus Selective Agar Base

CM0057D
RT避光干燥
3年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
CM0057D 250g $58.89
Product Name Bacilus Cereus Selective Agar Base
Introduction：

用途：用于蜡样芽胞杆菌的选择性分离培养。

添加剂：每瓶需配套添加12盒多粘菌素B和12盒50%卵黄乳液使用，每100ml蜡样芽孢杆菌选择性琼脂培养基基础中添加1支多粘菌素和2支50%卵黄乳液。

成分（g/L）：

胰酪蛋白胨：10.0

牛肉浸粉：1.0

D-甘露醇：10.0

氯化钠：10.0

苯酚红：0.025

琼脂：12.0

pH值7.2±0.2：25℃

检验原理：

胰酪蛋白胨、牛肉浸粉提供微生物生长的基本营养，D-甘露醇为唯一可发酵糖类，蜡样芽胞杆菌不发酵甘露醇，菌落一般为橙红色或粉红色，而发酵甘露醇的细菌因产酸而显黄色，蜡样芽胞杆菌可产生卵磷脂酶，分解卵黄，在菌落周围产生沉淀环；氯化钠维持均衡的渗透压；苯酚红作为酸碱指示剂；琼脂为凝固剂。

用法：

称取本品43.0g，加热溶解于1000ml蒸馏水中，分装每瓶100ml，121℃高压灭菌15分钟，冷至50℃左右时，每瓶加入10ml 50%的无菌卵黄乳液和多粘菌素B  10000IU，混匀，倾入无菌平皿，备用。

These protocols are for reference only. Biotopped does not independently validate these methods.

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