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YEB Agar Medium

Catalog No.： CM0071D/旧P40006D

Storage Condition： RT密封干燥

Shelf Life： 3年

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Catalog No.	Specification	List Price	Preferential Price	VIP	Beijing	Harbin	Shenyang	Guangzhou	Quantity	Cart
CM0071D/旧P40006D	500g	$82.56

Product Name

YEB Agar Medium

Introduction：

产品简介:

YEB 培养基用于农杆菌活化后单菌落扩增的培养。

成分组成:(g/L)

成分	YEB 琼脂
酵母粉	1
蛋白陈	5
牛肉粉	5
蔗糖	5
硫酸镁	0.24
琼脂粉	15
合计加入量	31.24
pH	7.0士0.2

配制方法:

1.按合计加入量取培养基，加入去离子水定容至 1000mL，搅拌均匀，调节 pH值至7.0士0.2

2.121℃高温灭菌 15 min 或 115℃高温灭菌 20 min

3.培养基冷却至 55℃左右，倾倒瓶皿，并置放于 4℃冰箱保存

注: 固体培养基调节 pH 后可以加热济解进行分装(琼脂低温不会解)一般情况下液体培养基不需要加热溶解和倾倒瓶皿。

注意事项:

1.注意无菌操作，避免微生物污染;根据菌株生长特性，可适当调节 pH 值。

2.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。

3.为了您的安全和健康，称量时注意粉尘，穿好实验服并佩戴一次性手套和口罩操作以避免引起呼吸道系统不适。

4.干粉培养基使用后立即旋紧瓶盖，避免吸潮结块，根据存放条件的不同，保质时间有一定的差异。

These protocols are for reference only. Biotopped does not independently validate these methods.

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YEB Agar Medium

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