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木质素选择性固体培养基

CM0084D
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CM0084D 500g $136.22
Product Name
Introduction：

这是指在天然固体物料（如秸秆）上补充的营养液成分，最终含水量控制在65-75%。

1. 固体物料：麦秸或玉米秸秆，粉碎至1-3cm长，50g (干重)

2. 碳源补充：葡萄糖或微晶纤维素，1.5g

3. 氮源（低氮关键）：酒石酸铵，0.22g (约相当于总氮0.05%)

4. 无机盐溶液成分 (溶于100mL蒸馏水)：

   · KH₂PO₄：1.0g

   · MgSO₄·7H₂O：0.5g

   · CaCl₂：0.1g

   · 微量元素母液：10 mL

5. 微量元素母液 (1L)：

   · 氨三乙酸：1.5g

   · MgSO₄·7H₂O：3.0g

   · MnSO₄·H₂O：0.5g

   · NaCl：1.0g

   · FeSO₄·7H₂O：0.1g

   · CoSO₄：0.1g

   · CaCl₂：0.1g

   · ZnSO₄·7H₂O：0.1g

   · CuSO₄·5H₂O：0.01g

   · AlK(SO₄)₂·12H₂O：0.01g

   · H₃BO₃：0.01g

   · 钼酸钠：0.01g

6. 诱导物 (提高选择性)：

   · 吐温80：0.5g (提高木质素过氧化物酶活性)

   · 可选：藜芦醇，0.05g

7. 缓冲剂：2,2-二甲基琥珀酸，1.0g (用NaOH调pH至4.5)

These protocols are for reference only. Biotopped does not independently validate these methods.

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