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PALCAM Medium

CM0093D
RT避光
3年
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Catalog No. Specification List Price Preferential Price VIP Beijing Harbin Shenyang Guangzhou Quantity Cart
CM0093D 250g $137.32
Product Name PALCAM Medium
Introduction：

用途：用于单增李斯特氏菌的选择性分离。

添加剂：

1、每瓶添加4盒PALCAM添加剂(A+B)使用；

2、每200ml PALCAM培养基添加一套 PALCAM添加剂(A+B)。

成分(g/L)：

酵母浸粉：8.0

葡萄糖：0.5

七叶苷：0.8

柠檬酸铁铵：0.5

甘露醇：10.0

酚红：0.1

氯化锂：15.0

酪蛋白胰酶消化物：10.0

心胰酶消化物：3.0

玉米淀粉：1.0

肉胃酶消化物：5.0

氯化钠：5.0

琼脂：15.0

pH值7.2±0.2：25℃

检验原理：

酪蛋白胰酶消化物酵母浸粉提供生长必须的碳氮源、维生素和生长因子；氯化钠可维持均衡的渗透压；甘露醇是可发酵的糖，酚红是pH指示剂；氯化锂和其它的抗生素能抑制革兰氏阴性菌和大多数革兰氏阳性菌；琼脂是培养基的凝固剂。

用法：

称取本品73.9g，加热搅拌溶解于1000ml蒸馏水中，分装三角瓶，每瓶200ml，121℃高压灭菌15分钟，冷至50℃左右时，加入1套PALCAM添加剂（添加剂1和添加剂2），混匀，倾入无菌平皿。

注：培养基中含柠檬酸铁铵、玉米淀粉，灭菌后可能出现微量沉淀。

These protocols are for reference only. Biotopped does not independently validate these methods.

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