Unit definition: One unit will oxidate one micromole of androsterone per min at pH 8.25 at 25℃.
Molecular weight: 28 kDa (SDS-PAGE)
Isoelectric point: 7.5
Michaelis constant: 3.0×10-5M(androsterone) 6.0×10-6M(NAD+)
Optimum pH: 10.5
Optimum temperature: 60℃
pH Stability: 4.5~10.5(30℃,20hr)
Thermal stability: < 50℃ (pH 7.2, 20min)
Inhibitors: Ag+,Hg2+,Cu2+,Zn2+,Fe3+
用途: 催化羟基类固醇的脱氢反应
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These protocols are for reference only. Biotopped does not independently validate these methods.